PREPARATION OF A MONOSPECIFIC ANTISERUM TO THYROXINE BINDING GLOBULIN FOR ITS QUANTITATION BY ROCKET IMMUNOELECTROPHORESIS

被引：51

作者：

BRADWELL, AR

BURNETT, D

RAMSDEN, DB

BURR, WA

PRINCE, HP

HOFFENBERG, R

机构：

[1] QUEEN ELIZABETH MED CTR, BIRMINGHAM B15 2TG, ENGLAND

[2] UNIV BIRMINGHAM, DEPT MED, BIRMINGHAM B15 2TT, ENGLAND

来源：

CLINICA CHIMICA ACTA | 1976年 / 71卷 / 03期

关键词：

D O I：

10.1016/0009-8981(76)90102-9

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A simple technique is described for the preparation of a high titer monospecific [sheep] antiserum to human serum thyroxine-binding globulin (TBG). This allows quantitation of the protein by rocket immunoelectrophoresis in 6 h with a day-to-day coefficient of variation of 2.9%. Using this technique serum concentrations of TBG were determined in groups of patients in good health, in pregnancy and in women on oral contraceptive therapy.

引用

页码：501 / 510

页数：10

共 15 条

[1]

BERNE BH, 1974, CLIN CHEM, V20, P61

[2] IMPROVED METHODOLOGY AND PRECISION USING A STRAIGHT BASE LINE TECHNIQUE FOR QUANTITATION OF PROTEINS BY 2 DIMENSIONAL IMMUNOELECTROPHORESIS [J].