BIOLOGICAL MONITORING OF ISOCYANATES AND RELATED AMINES .1. DETERMINATION OF 1,6-HEXAMETHYLENE DIAMINE (HDA) IN HYDROLYZED HUMAN-URINE AFTER ORAL-ADMINISTRATION OF HDA

被引:21
作者
BRORSON, T [1 ]
SKARPING, G [1 ]
SANDSTROM, JF [1 ]
STENBERG, M [1 ]
机构
[1] UNIV LUND,CTR CHEM,DEPT APPL NUTR,S-22100 LUND,SWEDEN
关键词
Gas chromatography; HDA; Mass spectrometry; N-acetylatoon phenotype; Urinary excretion;
D O I
10.1007/BF00397852
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
1,6-Hexamethylene diamine (HDA), used as raw material in industrial manufacturing operations, was orally administered to six healthy volunteers. After acid hydrolysis of the urine by hydrochloric acid, HDA and the metabolite 6-aminohexanoic acid were quantified. HDA was determined as an ethyl-chloroformate derivative by capillary gas chromatography using thermionic specific detection (TSD), and 6-aminohexanoic acid was quantified by ion chromatography using the ninhydrin reaction. In nonhydrolysed urine, monoacetylated HDA (N-acetyl-1,6-hexamethylene diamine) and HDA, were verified as heptafluorobutyric anhydride derivatives by gas chromatography-mass spectrometry (GC-MS), in a chemical ionization mode using isobutane and ammonia as reagent gases. In hydrolysed urine, a mean of 0.28 mg (range 1-6%) of the administered dose (8.2 mg) was recovered as HDA, and a mean of 0.8 mg (range < 1-27%) as 6-aminohexanoic acid. The urinary excretion of both the determined compounds was rapid, and the principal part (> 90%) of the elimination was completed within 10 h. There was a considerable inter-individual variation in the excreted amounts, but the intra-individual variation in the excretion of HDA was limited. The subjects N-acetylator phenotype was determined by a dapsone test. Three slow acetylators excreted lower amounts (mean 2% of given dose) of HDA than three rapid ones (mean 5%). © 1990 Springer-Verlag.
引用
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页码:79 / 84
页数:6
相关论文
共 16 条
  • [1] BEARD RR, 1981, PATTYS IND HYGIENE B, V2, P3135
  • [2] BERODE M, 1988, BIOCHEM INT, V17, P141
  • [3] THE EFFECTS OF FASTING ON THE ACUTE ORAL TOXICITY OF 9 CHEMICALS IN THE RAT
    DASHIELL, OL
    KENNEDY, GL
    [J]. JOURNAL OF APPLIED TOXICOLOGY, 1984, 4 (06) : 320 - 325
  • [4] LOCALIZATION OF 1,6-[C-14 DIAMINOHEXANE (HMDA) IN THE PROSTATE AND THE EFFECTS OF HMDA ON EARLY GESTATION IN FISCHER-344 RATS
    DAVID, RM
    HECK, HD
    [J]. TOXICOLOGY LETTERS, 1983, 17 (1-2) : 49 - 55
  • [5] GAS-CHROMATOGRAPHIC ANALYSIS OF METABOLITES OF THE CELL DIFFERENTIATING AGENT HEXAMETHYLENE BISACETAMIDE
    EGORIN, MJ
    ZUHOWSKI, EG
    NAYAR, MSB
    CALLERY, PS
    [J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 415 (01): : 148 - 155
  • [6] ACETYLATOR PHENOTYPING - A COMPARISON OF THE ISONIAZID AND DAPSONE TESTS
    HANSON, A
    MELANDER, A
    WAHLINBOLL, E
    [J]. EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY, 1981, 20 (03) : 233 - 234
  • [7] HEBY O, 1981, POLYAMINES BIOL MED, P243
  • [8] SUBCHRONIC INHALATION TOXICITY OF HEXAMETHYLENEDIAMINE IN RATS
    JOHANNSEN, FR
    LEVINSKAS, GJ
    BENDYKE, R
    HOGAN, GK
    [J]. FUNDAMENTAL AND APPLIED TOXICOLOGY, 1987, 9 (03): : 504 - 511
  • [9] EFFECTS OF ALIPHATIC DIAMINES ON RAT-LIVER ORNITHINE DECARBOXYLASE ACTIVITY
    PEGG, AE
    CONOVER, C
    WRONA, A
    [J]. BIOCHEMICAL JOURNAL, 1978, 170 (03) : 651 - 660
  • [10] DETERMINATION IN URINE OF DIISOCYANATE-DERIVED AMINES FROM OCCUPATIONAL EXPOSURE BY GAS-CHROMATOGRAPHY MASS FRAGMENTOGRAPHY
    ROSENBERG, C
    SAVOLAINEN, H
    [J]. ANALYST, 1986, 111 (09) : 1069 - 1071