GENETIC-TRANSFORMATION OF INTACT-CELLS OF LACTOBACILLUS-CURVATUS LC2-C AND LACT-SAKE LS2 BY ELECTROPORATION

被引：24

作者：

GAIER, W ^{[1
]}

VOGEL, RF ^{[1
]}

HAMMES, WP ^{[1
]}

机构：

[1] UNIV HOHENHEIM,INST FOOD TECHNOL,W-7000 STUTTGART 70,GERMANY

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1990年 / 11卷 / 02期

关键词：

D O I：

10.1111/j.1472-765X.1990.tb01280.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An electroporation‐induced transformation system was developed for two raw sausage starter organisms: Lactobacillus curvatus Lc2‐c and Lact. sake Ls2. Several plasmids of different origin (pAMβ1, pIL253, pNZ12 and pLP825) were tested for transformation and expression of their resistance determinants. Transfer rates were strongly dependent on growth phase and voltage. Several parameters were studied with possible influence on trasformation efficiencies. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：81 / 83

页数：3

共 12 条

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