TIME-RESOLVED FLUOROMETRY OF PURPLE MEMBRANE OF HALOBACTERIUM-HALOBIUM - O(640) AND AN O-LIKE RED-SHIFTED INTERMEDIATE-Q

被引：19

作者：

OHTANI, H ^{[1
]}

ITOH, H ^{[1
]}

SHINMURA, T ^{[1
]}

机构：

[1] HAMAMATSU PHOTON KK,TSUKUBA RES LAB,TSUKUBA,IBARAKI 30012,JAPAN

来源：

FEBS LETTERS | 1992年 / 305卷 / 01期

关键词：

BACTERIORHODOPSIN; PURPLE MEMBRANE; PHOTOCYCLE OF N(560); FLUORESCENCE OF O(640); Q INTERMEDIATE; HALOBACTERIUM-HALOBIUM;

D O I：

10.1016/0014-5793(92)80643-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The photocycle of the light-adapted purple membrane was studied with a time-resolved fluorometry apparatus: fluorescence of the sample suspension (>660 nm) was pumped with a 633-nm cw laser and the temporal change induced by a 532-nm pulsed laser was measured with a photon-counting-type transient recorder. The formation and the decay of the O640 intermediate were clearly observed in the pH region between 4.0 and 11.4. A photochemical cycle of N560 was apparently driven in alkaline suspension (pH > 9.3). An O-like fluorescent intermediate Q appears and decays with time constants of <0.1 ms and 1.7 +/- 0.2 ms, respectively.

引用

页码：6 / 8

页数：3

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