P-32 POSTLABELING OF DNA-ADDUCTS OF STYRENE-EXPOSED LAMINATION WORKERS

被引：55

作者：

VODICKA, P ^{[1
]}

VODICKOVA, L ^{[1
]}

HEMMINKI, K ^{[1
]}

机构：

[1] KAROLINSKA INST,CTR NUTR & TOXICOL,NOVUM,S-14157 HUDDINGE,SWEDEN

来源：

CARCINOGENESIS | 1993年 / 14卷 / 10期

关键词：

D O I：

10.1093/carcin/14.10.2059

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Lamination workers are exposed to high concentrations of styrene. A postlabeling method was developed for the detection of styrene-specific DNA adducts of the workers. To synthesize a standard, styrene oxide was reacted with 2'-deoxyguanosine 3'-monophosphate (dGMP) and the O6-dGMP-adduct was isolated and characterized. The human samples were assayed by the nuclease P1 version of the P-32-postlabeling technique, using magnet transfer of the adducts in chromatography. The human samples were spiked with the standards to ensure identification and quantitation. In lamination workers the O6-adducts, adjusted for adduct recovery, were detected at a level of 5 adducts/10(8) nucleotides, over five times the level in the controls.

引用

页码：2059 / 2061

页数：3

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