HEPATOCYTE GROWTH-FACTOR INHIBITS INTERCELLULAR COMMUNICATION VIA GAP-JUNCTIONS IN RAT HEPATOCYTES

被引：27

作者：

IKEJIMA, K ^{[1
]}

WATANABE, S ^{[1
]}

KITAMURA, T ^{[1
]}

HIROSE, M ^{[1
]}

MIYAZAKI, A ^{[1
]}

SATO, N ^{[1
]}

机构：

[1] JUNTENDO UNIV,SCH MED,DEPT GASTROENTEROL,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 214卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.2306

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrated that HGF reduces gap junctional intercellular communication of rat hepatocyte doublers and increases cell spreading. Intercellular communication via gap junctions was markedly reduced in hepatocyte doublets with HGF (20 ng/ml) 3-12 hr after inoculation. Immunocytochemistry revealed spotty localization of connexin 32 on the plasma membrane of 3-hr cultured control hepatocyte doublers, whereas HGF-treated doublers showed significantly less connexin 32 staining. Genistein maintained intercellular communication in the presence of HGF, indicating that HGF reduces intercellular communication by down regulation of connexin 32 through the c-met/HGF receptor-tyrosine-kinase-mediated pathway. Furthermore, TGF-beta(1) maintained intercellular communication in the presence of HGF. Regulation of intercellular communication in hepatocytes by HGF and TGF-beta(1) might play an important role during liver regeneration. (C) 1995 Academic Press, Inc.

引用

页码：440 / 446

页数：7

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