PRODUCTION OF ANTIBODY BY HUMAN B-CELLS UNDER SERUM-FREE CONDITIONS

被引：32

作者：

FARRANT, J ^{[1
]}

NEWTON, CA ^{[1
]}

NORTH, ME ^{[1
]}

WEYMAN, C ^{[1
]}

BRENNER, MK ^{[1
]}

机构：

[1] MIDDLESEX HOSP,SCH MED,COURTAULD INST BIOCHEM,LONDON W1P 7PN,ENGLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1984年 / 68卷 / 1-2期

关键词：

D O I：

10.1016/0022-1759(84)90133-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：25 / 34

页数：10

共 15 条

[1]

ARMITAGE P, 1971, STATISTICAL METHODS

[2]

BRENNER MK, 1983, IMMUNOLOGY, V50, P377

[3]

BRENNER MK, 1981, CELL IMMUNOL, V57, P280

[4] SPECIFIC INVITRO ANTIBODY-RESPONSE TO INFLUENZA-VIRUS BY HUMAN-BLOOD LYMPHOCYTES [J].

CALLARD, RE .

NATURE, 1979, 282 (5740) :734-736

[5]

CHEN RF, 1967, J BIOL CHEM, V242, P173

[6]

FARRANT J, 1981, CLIN EXP IMMUNOL, V45, P504

[7] CONDITIONS FOR MEASURING DNA-SYNTHESIS IN PHA STIMULATED HUMAN-LYMPHOCYTES IN 20 MU-L HANGING DROPS WITH VARIOUS CELL CONCENTRATIONS AND PERIODS OF CULTURE [J].

FARRANT, J ;

CLARK, JC ;

LEE, H ;

KNIGHT, SC ;

OBRIEN, J .

JOURNAL OF IMMUNOLOGICAL METHODS, 1980, 33 (04) :301-312

[8] COMPLETE REPLACEMENT OF SERUM IN PRIMARY CULTURES OF ERYTHROPOIETIN-DEPENDENT RED-CELL PRECURSORS (CFU-E) BY ALBUMIN, TRANSFERRIN, IRON, UNSATURATED FATTY-ACID, LECITHIN AND CHOLESTEROL [J].

ISCOVE, NN ;

GUILBERT, LJ ;

WEYMAN, C .

EXPERIMENTAL CELL RESEARCH, 1980, 126 (01) :121-126

[9] COMPLETE REPLACEMENT OF SERUM BY ALBUMIN, TRANSFERRIN, AND SOYBEAN LIPID IN CULTURES OF LIPOPOLYSACCHARIDE-REACTIVE B-LYMPHOCYTES [J].

ISCOVE, NN ;

MELCHERS, F .

JOURNAL OF EXPERIMENTAL MEDICINE, 1978, 147 (03) :923-933

[10] SIMPLE HANGING DROP (20 MU-L) DOUBLE ANTIBODY-RADIOIMMUNOASSAY OF HUMAN-IGM, IGG, IGA, IGD AND IGE [J].

NEWTON, CA ;

PEREIRA, RS ;

FARRANT, J .

JOURNAL OF IMMUNOLOGICAL METHODS, 1981, 45 (01) :41-49

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