HAB-1, A NEW HETEROMYELOMA FOR CONTINUOUS PRODUCTION OF HUMAN MONOCLONAL-ANTIBODIES

被引：16

作者：

FALLER, G ^{[1
]}

VOLLMERS, HP ^{[1
]}

WEIGLEIN, I ^{[1
]}

MARX, A ^{[1
]}

ZINK, C ^{[1
]}

PFAFF, M ^{[1
]}

MULLERHERMELINK, HK ^{[1
]}

机构：

[1] UNIV WURZBURG,INST PATHOL,JOSEF SCHNEIDER STR 2,W-8700 WURZBURG,GERMANY

来源：

BRITISH JOURNAL OF CANCER | 1990年 / 62卷 / 04期

关键词：

D O I：

10.1038/bjc.1990.336

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To obtain suitable cell lines for the immortalisation of human lymphocytes, we constructed a heteromyeloma between the murine myeloma Ag8 and human lymphocytes from a highly malignant polymorphic, centroblastic B-cell lymphoma. The thioguanine-resistant and HAT-sensitive heteromyeloma HAB-1 neither secretes nor contains cytoplasmatic immunoglobulins, the cells being EBV negative but positively stained for HLA-BC and the human proliferation marker Ki-67. The karyotype consists of about 50 murine and 20 human chromosomes. The HAB-1 cells grow in suspension and have a doubling rate of about 25-30 h. In fusion experiments with spleen cells from stomach carcinoma patients HAB-1 cells show a 5-7 times higher fusion efficiency than murine Ag8 cells or another heteromyeloma SPM4-0 and give stable antibody producing products. The cell line will be made available to interested scientists. © Macmillan Press Ltd., 1990.

引用

页码：595 / 598

页数：4

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