NUCLEOTIDE-SEQUENCE AND CHARACTERIZATION OF A CDNA-ENCODING THE ACETOHYDROXY ACID ISOMEROREDUCTASE FROM ARABIDOPSIS-THALIANA

被引:12
作者
CURIEN, G [1 ]
DUMAS, R [1 ]
DOUCE, R [1 ]
机构
[1] CNRS, UNITE MIXTE, RHONE POULENC AGROCHIM, 14-20 RUE PIERRE BAIZET, F-69263 LYON 09, FRANCE
关键词
ACETOHYDROXY ACID ISOMEROREDUCTASE; ARABIDOPSIS-THALIANA; CDNA SEQUENCE; TRANSIT PEPTIDE;
D O I
10.1007/BF00014556
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The primary structure of acetohydroxy acid isomeroreductase from Arabidopsis thaliana was deduced from two overlapping cDNA. The full-length cDNA sequence predicts an amino acid sequence for the protein precursor of 591 residues including a putative transit peptide of 67 amino acids. Comparison of the A. thaliana and spinach acetohydroxy acid isomeroreductases reveals that the sequences are conserved in the mature protein regions, but divergent in the transit peptides and around their putative processing site.
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页码:717 / 722
页数:6
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