COMPLEMENTATION OF THE PROTEIN-TRANSPORT DEFECT OF AN ESCHERICHIA-COLI SEC-Y MUTANT (SECY24) BY BACILLUS-SUBTILIS-SEC-Y HOMOLOG

被引:28
作者
NAKAMURA, K
TAKAMATSU, H
AKIYAMA, Y
ITO, K
YAMANE, K
机构
[1] UNIV TSUKUBA,INST BIOL SCI,SAKURA,IBARAKI 305,JAPAN
[2] KYOTO UNIV,INST VIRUS RES,KYOTO 606,JAPAN
关键词
Bacillus subtilis; Functional conservation; Protein export; SecY homologue;
D O I
10.1016/0014-5793(90)81054-R
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bacillus subtilis SecY homologue shares 41.3% homology with that of E. coli and remarkably higher homologous regions (more than 80%) are present in the four cytoplasmic regions [(1990) J. Biochem. 107, 603-607]. Based on the formation of the mature form of OmpA in E. coli, we have shown that the protein transport defect of the E. coli sec Y mutant (secY24) is complemented by the gene product from the B. subtilis sec Y homologue, which is expressed under the lac promoter control. However, B. subtilis SecY could not restore growth of the E. coli mutant at non-permissive temperature. © 1990.
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页码:75 / 78
页数:4
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