ONE-STEP SEPARATION OF HUMAN FETAL LYMPHOCYTES FROM NUCLEATED RED-BLOOD-CELLS

被引：16

作者：

BHAT, NM ^{[1
]}

BIEBER, MM ^{[1
]}

TENG, NNH ^{[1
]}

机构：

[1] STANFORD UNIV,MED CTR,SCH MED,DEPT OBSTET & GYNECOL,STANFORD,CA 94305

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 131卷 / 01期

关键词：

Density gradient centrifugation; Fetal lymphocyte; Nucleated red blood cell;

D O I：

10.1016/0022-1759(90)90245-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a one step discontinuous density gradient centrifugation procedure to obtain a 20-fold enrichment of human fetal lymphoid cells, reducing contamination from nucleated red blood cells to less than 1%. © 1990.

引用

页码：147 / 149

页数：3

共 7 条

[1] IMMUNOCYTOCHEMICAL CHARACTERIZATION OF LYMPHOCYTE DEVELOPMENT IN HUMAN-EMBRYONIC AND FETAL LIVERS
ABE, J
[J]. CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1989, 51 (01): : 13 - 21
[2] BOFILL M, 1985, J IMMUNOL, V134, P1531
[3] SINGLE-STEP SEPARATION OF RED BLOOD-CELLS, GRANULOCYTES AND MONONUCLEAR LEUKOCYTES ON DISCONTINUOUS DENSITY GRADIENTS OF FICOLL-HYPAQUE
ENGLISH, D
ANDERSEN, BR
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1974, 5 (03) : 249 - 252
[4] LANDESBERG R, 1988, IMMUNOLOGY, V65, P323
[5] ROSENTHAL P, 1983, J IMMUNOL, V31, P232
[6] Trolle D., 1947, ACTA OBSTET ET GYNECOL SCAND, V27, P327
[7] VACCA LL, 1985, LABORATORY MANUAL HI, P109

← 1 →