A DNA PRIMER PROBE SYSTEM FOR THE RAPID AND SENSITIVE DETECTION OF MYCOBACTERIUM-TUBERCULOSIS-COMPLEX PATHOGENS

被引：8

作者：

RALPHS, NT ^{[1
]}

GARRETT, S ^{[1
]}

MORSE, R ^{[1
]}

COOKSON, JB ^{[1
]}

ANDREW, PW ^{[1
]}

BOULNOIS, GJ ^{[1
]}

机构：

[1] GLENFIELD GEN HOSP,LEICESTER,ENGLAND

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1991年 / 70卷 / 03期

关键词：

D O I：

10.1111/j.1365-2672.1991.tb02928.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

BamHI restriction fragment from Mycobacterium tuberculosis was found to hybridize specifically with genomic DNA from M. tuberculosis-complex organisms. Primers were designed from the terminal sequences of this fragment and used to amplify uniquely M. tuberculosis-group DNA in a polymerase chain reaction. It is suggested that a combination of these primers and probe will prove a useful tool for the early diagnosis of tuberculosis infections.

引用

页码：221 / 226

页数：6

共 32 条

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BAESS I, 1974, ACTA PATH MICRO IM B, VB 82, P780

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