A DNA PRIMER PROBE SYSTEM FOR THE RAPID AND SENSITIVE DETECTION OF MYCOBACTERIUM-TUBERCULOSIS-COMPLEX PATHOGENS

被引:8
作者
RALPHS, NT [1 ]
GARRETT, S [1 ]
MORSE, R [1 ]
COOKSON, JB [1 ]
ANDREW, PW [1 ]
BOULNOIS, GJ [1 ]
机构
[1] GLENFIELD GEN HOSP,LEICESTER,ENGLAND
来源
JOURNAL OF APPLIED BACTERIOLOGY | 1991年 / 70卷 / 03期
关键词
D O I
10.1111/j.1365-2672.1991.tb02928.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
BamHI restriction fragment from Mycobacterium tuberculosis was found to hybridize specifically with genomic DNA from M. tuberculosis-complex organisms. Primers were designed from the terminal sequences of this fragment and used to amplify uniquely M. tuberculosis-group DNA in a polymerase chain reaction. It is suggested that a combination of these primers and probe will prove a useful tool for the early diagnosis of tuberculosis infections.
引用
收藏
页码:221 / 226
页数:6
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