REAGENT FOR INTRODUCING PYRENE RESIDUES IN OLIGONUCLEOTIDES

被引：27

作者：

KORSHUN, VA ^{[1
]}

PESTOV, NB ^{[1
]}

BIRIKH, KR ^{[1
]}

BERLIN, YA ^{[1
]}

机构：

[1] MM SHEMYAKIN BIOORGAN CHEM INST, MIKLUKHO MAKLAYA 16-10, MOSCOW 117871, USSR

来源：

BIOCONJUGATE CHEMISTRY | 1992年 / 3卷 / 06期

关键词：

D O I：

10.1021/bc00018a016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel pyrenyl-containing phosphoramidite reagent,N-[4-(1-pyrenyl)butyryl]-O1-(4,4'-dimethoxytrityl)-O2-[(diisopropylamino)(2-cyanoethoxy)phosphino]-3-amino-1,2-propanediol (5), has been synthesized from 4-(1-pyrenyl)butanoic acid in four steps with the 52% overall yield and used to incorporate pyrene residue(s) into oligonucleotides. Oligonucleotides 6 and 7, bearing one or two pyrenes at the 5'-terminus, have been prepared by means of that reagent, characterized with fluorescence spectra, and successfully used as primers in a polymerase chain reaction.

引用

页码：559 / 562

页数：4

共 19 条

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