DIFFERENTIAL-DIAGNOSIS OF HTLV-I AND HTLV-II INFECTIONS BY RESTRICTION ENZYME ANALYSIS OF NESTED PCR PRODUCTS

被引:57
作者
TUKE, PW [1 ]
LUTON, P [1 ]
GARSON, JA [1 ]
机构
[1] UNIV COLL & MIDDLESEX SCH MED,DEPT MED MICROBIOL,DIV VIROL,LONDON,ENGLAND
关键词
HTLV-I; HTLV-II; DIAGNOSIS; TYPING; POLYMERASE CHAIN REACTION; RESTRICTION ENZYME ANALYSIS;
D O I
10.1016/0166-0934(92)90065-L
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A 'nested' polymerase chain reaction (PCR) assay is described which is capable of detecting single copies of human T-cell lymphotropic virus (HTLV) in genomic DNA extracted from peripheral blood mononuclear cells (PBMCs). A single set of 'nested' oligonucleotide primers, based on the highly conserved tax/rex region of the viral genome, was able to detect both HTLV-I and HTLV-II proviral sequences in clinical samples of diverse geographical origins, from the United States, Great Britain, Japan, the Caribbean, Italy, Greece, Iraq and West Africa. Rapid discrimination between HTLV-I and HTLV-II infections was achieved by restriction enzyme analysis of unpurified second-round PCR products, even in those cases in which serological assays had failed to provide a definitive result. Over a 2-year period, a total of 53 HTLV infections (37 HTLV-I and 16 HTLV-II) were identified by this technique and complete concordance with serological typing, available in 41 cases, was observed.
引用
收藏
页码:163 / 174
页数:12
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