CYTOTOXICITY TO ENDOTHELIAL-CELLS BY SERA FROM AGED MRL/LPR/LPR MICE IS ASSOCIATED WITH AUTOIMMUNITY TO CELL-SURFACE HEPARAN-SULFATE

被引:8
作者
DIMITRIUBONA, A
MATIC, M
DING, WH
YANG, CP
FILLIT, H
机构
[1] Henry L. Schwartz Department of Geriatrics and Adult Development, Mount Sinai Medical Center, New York
来源
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY | 1995年 / 76卷 / 03期
关键词
D O I
10.1006/clin.1995.1121
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Vasculitis is an common clinical feature of systemic lupus erythematosus (SLE) in humans and in animal models of this disease. Humoral autoimmunity against endothelial cells has been previously demonstrated in SLE and other autoimmune disorders, but the precise cell surface antigenic targets involved in the initiation and progression of vascular injury are still essentially unknown. In the current studies, we demonstrate the presence of autoantibodies in the sera of MRL/lpr/lpr mice which bind endothelial cell surface antigens by ELISA and also cause complement-dependent cytotoxicity of these cells. These MRL/lpr/lpr sera induced complement-dependent cleavage and release of (SO4)-S-35-labeled material containing primarily cell surface heparan sulfate proteoglycans from these cells, and react with heparin (a glycosaminoglycan related to heparan sulfate) by ELISA and liquid-phase competitive inhibition ELISA. These data indicate that antiendothelial cell autoantibodies present in autoimmune MRL/lpr/lpr mice are directed at least in part against cell surface heparan sulfate proteoglycans. Autoantibodies to cell surface heparan endothelial cell injury in these animals through complement-dependent, autoimmune mechanisms. (C) 1995 Academic Press, Inc.
引用
收藏
页码:234 / 240
页数:7
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