PURIFICATION AND ANALYSIS OF THE ANTIGENICITY OF A 69 000 DA PROTEIN FROM BORDETELLA-PERTUSSIS

被引：17

作者：

GOULDKOSTKA, JL

BURNS, DL

BRENNAN, MJ

MANCLARK, CR

机构：

[1] Laboratory of Pertussis, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Bethesda

来源：

FEMS MICROBIOLOGY LETTERS | 1990年 / 67卷 / 03期

关键词：

Antigenicity analysis; Bordetella pertussis; Protein purification;

D O I：

10.1016/0378-1097(90)90010-N

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A purification scheme was devised for a 69-kDa outer membrane protein of Bordetella pertussis, a virulence-associated protein which may play a role in the pathogenesis of the organism. The protein was purified to apparent homogeneity by heating B. pertussis cells for 1 h at 60°C followed by DEAE-Sepharose and Affi-Gel Blue chromatography. Antibodies found in sera obtained from patients diagnosed as having pertussis reacted with this protein. This purification scheme should be useful for the production of the 69 kDa protein which is currently being evaluated as a pertussis vaccine candidate. © 1990.

引用

页码：285 / 290

页数：6

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