ESTABLISHMENT OF NEW HUMAN PROSTATIC-CANCER CELL-LINE (JCA-1)

被引：66

作者：

MURAKI, J

ADDONIZIO, JC

CHOUDHURY, MS

FISCHER, J

ESHGHI, M

DAVIDIAN, MM

SHAPIRO, LR

WILMOT, PL

NAGAMATSU, GR

CHIAO, JW

机构：

[1] NEW YORK MED COLL,DEPT PATHOL,VALHALLA,NY 10595

[2] NEW YORK MED COLL,DEPT MED,VALHALLA,NY 10595

[3] NEW YORK MED COLL,DEPT PEDIAT,DIV MED GENET,VALHALLA,NY 10595

[4] NEW YORK MED COLL,DEPT PATHOL,DIV MED GENET,VALHALLA,NY 10595

来源：

UROLOGY | 1990年 / 36卷 / 01期

关键词：

D O I：

10.1016/0090-4295(90)80319-I

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

The establishment of a new human prostatic cancer cell line is described. This cell line was derived from a poorly to moderately differentiated prostatic adenocarcinoma. It has been maintained in tissue culture for fourteen months and has been passed fifty-two times. This cell line has an ability to form colonies in soft agar suspension cultures, and also is transplantable to nude mice. Tumors grown in nude mice revealed a poorly differentiated adenocarcinoma with positive PSA staining. Acid phosphatase activity was detected in freeze-thawed cells by enzymatic assay. A karyotype analysis demonstrated aneuploidy with a model chromosomal number of 69 and six marker chromosomes. © 1989 Lederle Laboratories.

引用

页码：79 / 84

页数：6

共 11 条

[1] DEVELOPMENT OF 3T3-LIKE LINES FROM BALB/C MOUSE EMBRYO CULTURES - TRANSFORMATION SUSCEPTIBILITY TO SV40 [J].