REGIONAL DIFFERENCES IN BIOELECTRICAL PROPERTIES AND ANION SECRETION IN CULTURED EPITHELIA FROM RAT AND HUMAN MALE EXCURRENT DUCTS

被引:24
作者
CHAN, HC [1 ]
LAI, KB [1 ]
FU, WO [1 ]
CHUNG, YW [1 ]
CHAN, PSF [1 ]
WONG, PYD [1 ]
机构
[1] CHINESE UNIV HONG KONG, FAC MED, DEPT SURG, SHA TIN, HONG KONG
关键词
D O I
10.1095/biolreprod52.1.192
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Bioelectrical properties and anion secretion in cultured epithelia from different regions of rat and human male excurrent ducts were studied by measuring the short-circuit currents (I-SC). In all regions of the rat excurrent duct, Cl- secretion accounts for over 90% of the basal I-SC, although the magnitude varied in different regions. Cl- secretion was found to be mediated by a Cl-/HCO3- exchanger, an Na+/H+ exchanger, and an Na+/K+/2Cl(-) symport located on the basolateral side of the epithelial cells. Forskolin, an activator of adenylate cyclase, and ionomycin, a Ca2+ ionophore, were used to investigate the relative importance of cAMP and Ca2+ as intracellular messengers regulating Cl- secretion in different regions. It was found that in both species, the forskolin-evoked I-SC response was larger in the proximal end (efferent duct/caput epididymidis [rat/human, respectively]) than in the distal end (cauda/corpus epididymidis). The response to ionomycin in the rat cauda epididymidis (distal end) was larger than that in the efferent duct (proximal end); on the other hand, no significant difference in the ionomycin-induced I-SC was observed in the caput and the corpus regions from the human epididymis. Our results indicate that while the cAMP- and Ca2+-dependent pathways are both involved in regulating Cl- secretion in all regions along the male excurrent ducts in both species, a regional difference exists with respect to the relative importance of the two regulatory pathways involved in Cl- secretion along the male reproductive tract.
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页码:192 / 198
页数:7
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