COMPARATIVE AND QUANTITATIVE STUDY OF L-DOPA DECARBOXYLASE MESSENGER-RNA IN RAT NEURONAL AND NONNEURONAL TISSUES

被引：11

作者：

COGE, F ^{[1
]}

KRIEGERPOULLET, M ^{[1
]}

GROS, F ^{[1
]}

THIBAULT, J ^{[1
]}

机构：

[1] UNIV PARIS 12,NEUROBIOL MOLEC LAB,F-94010 CRETEIL,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90492-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The L-DOPA decarboxylase mRNA levels were determined by a sensitive S1 nuclease method in four organs and one tumor of adult rat. S1 mapping analysis, with probes corresponding to the mRNA coding region, whowed that this region is conserved in all L-DOPA decarboxylase mRNA of neuronal and non-neuronal tissues. The mRNA was not very abundant; its representation varies approximately from 0.00035% of total RNA in the mid brain to 0.013% of total mRNA in the pheochromocytoma. A strong correlation between mRNA level and enzyme amount was observed (correlation coefficient = 0.99). The results indicate that the level of mRNA is a primary factor determining the L-DOPA decarboxylase level. © 1990.

引用

页码：1006 / 1012

页数：7

共 19 条

[1]

ALBERT VR, 1987, J BIOL CHEM, V262, P9404

[2] ASSOCIATION OF DIAMINE OXIDASE AND ORNITHINE DECARBOXYLASE WITH MATURING CELLS IN RAPIDLY PROLIFERATING EPITHELIUM [J].

BAYLIN, SB ;

STEVENS, SA ;

SHAKIR, KMM .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 541 (03) :415-419

[3]

BOUCHARD S, 1981, J NEUROCHEM, V37, P781

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] CHARACTERIZATION OF DOPA DECARBOXYLASE MESSENGER-RNA IN RAT PHEOCHROMOCYTOMA [J].

BRUNEAU, G ;

KRIEGERPOULLET, M ;

COGE, F ;

BORRIVOLTATTORNI, C ;

GROS, F ;

THIBAULT, J .

BIOCHIMIE, 1990, 72 (01) :73-76

[6] IDENTITY OF DOPA DECARBOXYLASE AND 5-HYDROXYTRYPTOPHAN DECARBOXYLASE [J].

CHRISTENSON, JG ;

DAIRMAN, W ;

UDENFRIEND, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (02) :343-+

[7] DECREASE IN LIVER AROMATIC L-AMINO-ACID DECARBOXYLASE PRODUCED BY CHRONIC ADMINISTRATION OF L-DOPA [J].

DAIRMAN, W ;

CHRISTENSON, JG ;

UDENFRIEND, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (09) :2117-+

[8]

GOLSTEIN M, 1972, PHARMACOL REV, V24, P293

[9]

HADJICONSTANTINOU M, 1988, J NEUROCHEM, V51, P1560

[10] IMMUNOHISTOCHEMICAL LOCALIZATION OF AROMATIC L-AMINO-ACID DECARBOXYLASE (DOPA DECARBOXYLASE) IN CENTRAL DOPAMINE AND 5-HYDROXYTRYPTAMINE NERVE CELL BODIES OF RAT [J].

HOKFELT, T ;

FUXE, K ;

GOLDSTEIN, M .

BRAIN RESEARCH, 1973, 53 (01) :175-180

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