RECOMBINANT BACILLUS-THURINGIENSIS CRYSTAL PROTEINS WITH NEW PROPERTIES - POSSIBILITIES FOR RESISTANCE MANAGEMENT

被引：99

作者：

BOSCH, D

SCHIPPER, B

VANDERKLEIJ, H

DEMAAGD, RA

STIEKEMA, WJ

机构：

[1] Department of Molecular Biology, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Wageningen, 6700 AA

来源：

BIO-TECHNOLOGY | 1994年 / 12卷 / 09期

关键词：

D O I：

10.1038/nbt0994-915

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To obtain Bacillus thuringiensis crystal proteins with new properties and to identify the regions involved in insecticidal activity, we generated hybrid genes composed of cryIC and cryIE by in vivo recombination. Analysis of the hybrid proteins showed that domain III of CryIC is involved in the toxicity towards Spodoptera exigua and Mamestra brassicae. Transfer of this domain to CryIE, which is not active against these insects, resulted in a new protein with a broader activity. This hybrid protein binds to different receptors than CryIC, suggesting its use as an alternative for CryIC in resistance management programs.

引用

页码：915 / 918

页数：4

共 16 条

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