GENE SPECIFICITY IS MAINTAINED IN TRANSIENT EXPRESSION ASSAYS WITH PROTOPLASTS DERIVED FROM DIFFERENT TISSUES OF BARLEY

被引：5

作者：

DIAZ, I

ROYO, J

DELAHOZ, PS

CARBONERO, P

机构：

[1] Laboratorio de Bioquímica y Biología Molecular, Departamento de Biotecnología, E.T.S.I. Agrónomos-UPM, Madrid

[2] Dpto. de Biología Celular y Genética, Facultad de Ciencias, Universidad de Alcalá de Henares, Alcalá de Henares

来源：

EUPHYTICA | 1995年 / 85卷 / 1-3期

关键词：

BARLEY; ELECTROPORATION; PEG-MEDIATED DNA UPTAKE; PROMOTER ANALYSIS; PROTOPLASTS; TRANSIENT EXPRESSION;

D O I：

10.1007/BF00023949

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In some cereal species that are still recalcitrant to stable transformation and regeneration, transient expression in isolated protoplasts is a useful tool for the study of gene expression and regulation. We have successfully applied these techniques to barley protoplasts derived from developing endosperm, aleurone, leaves and roots in order to characterize functionally cis-acting motives in two gene promoters, corresponding to trypsin inhibitorBTI-CMe and to sucrose synthase Ss1. Gene specificity is maintained in transient expression assays with protoplasts isolated from these different barley tissues and the pattern of expression parallels the mRNA levels observed for the corresponding genes in the same tissues.

引用

页码：203 / 207

页数：5

共 19 条

[1]

DELAHOZ PS, 1992, THESIS U POLITECNICA

[2]

DIAZ I, 1992, PLANT CELL REP, V10, P595, DOI 10.1007/BF00232377

[3] OPTIMIZATION OF CONDITIONS FOR DNA UPTAKE AND TRANSIENT GUS EXPRESSION IN PROTOPLASTS FOR DIFFERENT TISSUES OF WHEAT AND BARLEY [J].