DETERMINATION OF THE PHYSICOCHEMICAL CONSTANTS AND SPECTROPHOTOMETRIC CHARACTERISTICS OF THE METALLOCHROMIC ZINCON AND ITS POTENTIAL USE IN BIOLOGICAL-SYSTEMS

被引：18

作者：

HILARIO, E ^{[1
]}

ROMERO, I ^{[1
]}

CELIS, H ^{[1
]}

机构：

[1] NATL AUTONOMOUS UNIV MEXICO,INST FISIOL CELULAR,DEPT BIOENERGET,APARTADO POSTAL 70600,MEXICO CITY 04510,DF,MEXICO

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1990年 / 21卷 / 03期

关键词：

Divalent cation; Membrane; Metallochromic; Spectrophotometry; Zinc ion; Zincon;

D O I：

10.1016/0165-022X(90)90013-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work we report a detailed characterization of the metallochromic Zincon. Zincon forms complexes with Zn2+ and Cu2+, producing change in colour; the complexes with Fe2+, Mn2+ and Ca2+ cause the bleaching of the Zincon solutions. Mg2+ does not interact with Zincon nor does it change its spectral characteristics. The presence of Ca2+ and Mg2+ does not interface with the spectral characteristics of the Zn-Zincon complex. The Kd, Ks and Δε{lunate} values for the complexes were determined. The Δε{lunate} values were very high, making this spectrophotometric method very sensitive. The complexes Zn-Zincon is fully reversible; however, the complex Cu-Zincon in partially reversible. The free-Zincon, and the complexes Zn-Zincon and Cu-Zincon, does not partition into organic solvents, does not permeate liposome membrane, and neither does it interact with biological membranes. All these characteristics make the metallochronic Zincon useful in biological systems. © 1990.

引用

页码：197 / 207

页数：11

共 18 条

[1] THE PHOSPHATE PYROPHOSPHATE EXCHANGE AND HYDROLYTIC REACTIONS OF THE MEMBRANE-BOUND PYROPHOSPHATASE OF RHODOSPIRILLUM-RUBRUM - EFFECTS OF PH AND DIVALENT-CATIONS [J].