ENHANCEMENT OF ENZYMATIC PRODUCTION OF CYCLODEXTRINS BY ORGANIC-SOLVENTS

被引：29

作者：

LEE, YD ^{[1
]}

KIM, HS ^{[1
]}

机构：

[1] KOREA INST SCI & TECHNOL, DEPT BIOL SCI & ENGN, POB 150, CHEONGYANG, SEOUL 130650, SOUTH KOREA

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1991年 / 13卷 / 06期

关键词：

CYCLODEXTRIN GLYCOSYLTRANSFERASE (CGTASE); CYCLODEXTRINS; DEXTRINIZING ACTIVITY; ORGANIC SOLVENTS; BACILLUS SP;

D O I：

10.1016/0141-0229(91)90009-Y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A strain of Bacillus sp.BE101 was isolated from soil and used to obtain the cyclodextrin glycosyltransferase (E.C.3.2.1.19, CGTase). Cyclodextrin glycosyltransferase from Bacillus sp. BE101 synthesized mainly beta-cyclodextrin from soluble starch; the composition of alpha-, beta-, and gamma-cyclodextrin was 1 : 7 : 2. The optimum conditions for CGTase activity were pH 6.0-6.5 and temperature 45-degrees-C. An increase in cyclodextrin production by organic solvents such as acetone, propanol, and ethanol was observed. The maximum increase was observed at 10% ethanol, and the enhancement of production yield was about 100%. Studies on the cause of the increase in production yield of cyclodextrins by ethanol were carried out. It seemed that enhancement of the yield by ethanol was due to the increase in hydrolysis of starch by CGTase in the presence of ethanol.

引用

页码：499 / 503

页数：5

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