ONE-STEP IMMUNOAFFINITY PURIFICATION OF COMPLEX-I SUBUNITS FROM BEEF-HEART MITOCHONDRIA

被引：3

作者：

HAINES, AMR

COOPER, JM

MORGANHUGHES, JA

CLARK, JB

SCHAPIRA, AHV

机构：

[1] INST NEUROL, DEPT CLIN NEUROL, LONDON WC1N 3BG, ENGLAND

[2] INST NEUROL, DEPT NEUROCHEM, LONDON WC1N 3BG, ENGLAND

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1992年 / 3卷 / 03期

关键词：

D O I：

10.1016/1046-5928(92)90018-R

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polypeptides of beef heart mitochondrial complex I were isolated from 15 mg of solubilized beef heart mitochondria using antibodies immobilized on an agarose chromatography column. The preparation was examined by SDS electrophoresis and Western blotting using affinity-purified antibodies to complex I and compared to beef heart complex I purified according to the conventional method of Hatefi and Rieske. There was a high degree of homology between the two preparations as judged by SDS-polyacrylamide electrophoresis and by immunoblotting with seven affinity-purified antibodies to various complex I subunits. This method could be applied to the preparation of complex I subunits from small samples such as human muscle biopsy specimens. © 1992.

引用

页码：223 / 227

页数：5

共 18 条

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