INDUCTION OF C(4)-DICARBOXYLATE TRANSPORT GENES BY EXTERNAL STIMULI IN RHIZOBIUM-MELILOTI

被引：11

作者：

BATISTA, S ^{[1
]}

CASTRO, S ^{[1
]}

AGUILAR, OM ^{[1
]}

MARTINEZDRETS, G ^{[1
]}

机构：

[1] NATL UNIV LA PLATA,FAC CIENCIAS EXACTAS,DIV BIOCHEM 1,RA-1900 LA PLATA,ARGENTINA

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1992年 / 38卷 / 01期

关键词：

RHIZOBIUM-MELILOTI; NITROGEN FIXATION; DICARBOXYLATE TRANSPORT;

D O I：

10.1139/m92-008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have characterized two mutants of Rhizobium meliloti L5-30 obtained by random mutagenesis using Mu-lacZ that were defective in transport of C4-dicarboxylic acids. These mutants induced ineffective nodules on alfalfa. Mutations in the two strains appeared to be located in dctA gene. Levels of dctA gene expression were determined under different environmental conditions using dctA-lacZ fusions, and beta-galactosidase activities increased in response to osmotic stress and also when the cells were incubated in medium low in calcium. The transcriptional induction of the dctA gene by environmental signals was decreased by DNA gyrase inhibitors such as novobiocin and coumermycin.

引用

页码：51 / 55

页数：5

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