PERMSELECTIVE LIPID POLY(O-PHENYLENEDIAMINE) COATINGS FOR AMPEROMETRIC BIOSENSING OF GLUCOSE

Highly selective biosensing of glucose is achieved by entrapping glucose oxidase into a composite poly(o-phenylenediamine)(PPD)-lipid layer. The composite layer, resulting from such immobilization scheme, combines the size-exclusion sieving of PPD films with the hydrophobicity of lipid coatings. While maintaining highly sensitive and fast response towards the target glucose substrate, it strongly influences the transport and response of potential electroactive interferents. A dramatic improvement in the discrimination against ascorbic and uric acids and acetaminophen is thus accomplished. Factors affecting the behavior of the PPD-lipid-enzyme layer are elucidated and the analytical advantages are illustrated under batch and flow conditions.