PREPARATION AND PROPERTIES OF A MICRO ENZYME SENSOR FOR CREATINE

被引：9

作者：

MOTONAKA, J ^{[1
]}

TAKABAYASHI, H ^{[1
]}

IKEDA, S ^{[1
]}

TANAKA, N ^{[1
]}

机构：

[1] NIPPON JIMUKI CO LTD,CHIYODA KU,TOKYO 101,JAPAN

来源：

ANALYTICAL LETTERS | 1990年 / 23卷 / 11期

关键词：

ENZYME SENSOR; DETERMINATION OF CREATINE; CREATINE AMIDINOHYDROLASE; SARCOSINE OXIDASE;

D O I：

10.1080/00032719008052542

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A micro enzyme sensor using creatine amidinohydrolase (EC 3.5.3.3) and sarcosine oxidase (EC 1.5.3.1) was developed for measuring creatine. The sensor consisted of platinum and silver wires fixed in a glass tubing with epoxy resin adhesive, and creatine amidinohydrolase and sarcosine oxidase immobilized on their tip by cross-linking method. The calibration curve for measurement of creatine, the effects of pH, temperature, the amount of flavin adenine dinucleotide and compounds on the sensitivity, and the life-time of the sensor were examined. Under optimal conditions, the response of the sensor was linear in the concontration range of 10-mu-M approximately mM creatine.

引用

页码：1981 / 1991

页数：11