HAIRY (RAMIFIED) REGIONS OF PECTINS .2. RHAMNOGALACTURONASE - A NOVEL ENZYME THAT DEGRADES THE HAIRY REGIONS OF PECTINS

被引：133

作者：

SCHOLS, HA ^{[1
]}

GERAEDS, CCJM ^{[1
]}

SEARLEVANLEEUWEN, MF ^{[1
]}

KORMELINK, FJM ^{[1
]}

VORAGEN, AFJ ^{[1
]}

机构：

[1] AGR UNIV WAGENINGEN, DEPT FOOD SCI, BOMENWEG 2, 6703 HD WAGENINGEN, NETHERLANDS

来源：

CARBOHYDRATE RESEARCH | 1990年 / 206卷 / 01期

关键词：

D O I：

10.1016/0008-6215(90)84010-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A rhamnogalacturonase (RGase), that could degrade the modified hairy regions (MHR) prepared from apple cell walls, was isolated and purified from a technical preparation of Aspergillus aculeatus. The RGase cleaved galactopyranosyluronic-rhamnopyranosyl linkages. No activity was observed towards other cell-wall polysaccharides or p-nitrophenyl glycosides. The optimal conditions for RGase were pH 3-4 and 40-50°. MHR was degraded by RGase, and methylation analysis and 13C-n.m.r. spectroscopy indicated the products to have a tetrasaccharide backbone of alternating rhamnose and galacturonic acid residues. Some oligomers had a galactose residue 4-linked to rhamnose. The potential value of this new enzyme is considered. © 1990.

引用

页码：105 / 115

页数：11

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