DIETARY-CHOLESTEROL ENHANCES PRENEOPLASTIC ABERRANT CRYPT FORMATION AND ALTERS CELL-PROLIFERATION IN THE MURINE COLON TREATED WITH AZOXYMETHANE

被引：19

作者：

KENDALL, CW

JANEZIC, SA

FRIDAY, D

RAO, AV

机构：

[1] Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto

来源：

NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL | 1992年 / 17卷 / 02期

关键词：

D O I：

10.1080/01635589209514178

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The effect of dietary cholesterol on the development of colonic preneoplastic aberrant crypts, as well as its influence on the proliferative status of the intestinal epithelium, was investigated in mice exposed to the chemical carcinogen azoxymethane. Two strains of mice, C57BL/6J and BALB/cJ, were fed a semisynthetic diet containing 0% (control), 1.25%, or 5.00% cholesterol for eight weeks. During the first four weeks of the experiment, mice were given weekly injections of azoxymethane. Cholesterol supplementation significantly increased the formation of aberrant crypts (p < 0.0001), enhanced the rate of cell proliferation (p < 0.0001), altered the cell proliferative pattern, and increased crypt height (p < 0. 05) and the total number of cells per crypt (p < 0.01) in the colonic epithelium of both mouse strains. C57BL/6J mice developed a greater number of aberrant crypts (p < 0.0001). However, a diet-strain interaction was not observed. The results of this study indicate that dietary cholesterol enhances colon carcinogenesis in the murine colon and therefore may be an important factor in the etiology of large bowel cancer in humans.

引用

页码：107 / 114

页数：8

共 43 条

[1]

McKeown-Eyssen G.E., Bright-See E., Dietary Factors in Colon Cancer: International Relationships., Nutr Cancer, 6, pp. 160-170, (1984)

[2]

Haenszel W., Kurihara M., Studies of Japanese Migrants. I. Mortality from Cancer and Other Diseases Among Japanese in the United States., JNCI, 40, pp. 43-68, (1968)

[3]

Miller A.B., Howe G.R., Jain M., Craib K.J.P., Harrison L., Food Items and Food Groups as Risk Factors in a Case-Control Study of Diet and Colo-Rectal Cancer., Int J Cancer, 32, pp. 155-161, (1983)

[4]

Chen H.W., Kandutsch A.A., Heiniger H.J., The Role of Cholesterol in Malignancy, Prog Exp Tumor Res, 22, pp. 275-316, (1978)

[5]

Hiramatsu Y., Takada H., Yamamura M., Hioki K., Saito K., Et al., Effect of Dietary Cholesterol on Azoxymethane-Induced Colon Carcinogenesis in Rats., Carcinogenesis, 4, pp. 553-558, (1983)

[6]

Klurfeld D.M., Aglow E., Tepper S.A., Kritchevsky D., Modification of Dimethylhydrazine-Induced Carcinogenesis in Rats by Dietary Cholesterol., Nutr Cancer, 5, pp. 16-25, (1983)

[7]

Bruce W.R., Recent Hypothesis for the Origin of Colon Cancer., Cancer Res, 47, pp. 4237-4242, (1987)

[8]

Shinitzky M., Shaharabani E., Skornick Y., Possible Correlation Between Tumour Invasiveness and Low Serum Cholesterol., Cancer Detect Prev, 11, pp. 157-161, (1988)

[9]

Liu K., Stamler J., Moss D., Garside D., Persky V., Et al., Dietary Cholesterol, Fat and Fibre, and Colon Cancer Mortality. An Analysis of International Data., Lancet, 1, pp. 752-755, (1979)

[10]

Jain M., Cook G.M., Davis F.G., Grace M.G., Howe G.R., Et al., A Case-Control Study of Diet and Colorectal Cancer., Int J Cancer, 26, pp. 757-768, (1980)

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