AMINO-ACID-SEQUENCE AND EXPRESSION OF THE HEPATIC GLYCOGEN-BINDING (G(L))-SUBUNIT OF PROTEIN PHOSPHATASE-1

被引：132

作者：

DOHERTY, MJ ^{[1
]}

MOORHEAD, G ^{[1
]}

MORRICE, N ^{[1
]}

COHEN, P ^{[1
]}

COHEN, PTW ^{[1
]}

机构：

[1] UNIV DUNDEE, DEPT BIOCHEM, MRC, PROT PHOSPHORYLAT LAB, DUNDEE DD1 4HN, SCOTLAND

来源：

FEBS LETTERS | 1995年 / 375卷 / 03期

基金：

英国医学研究理事会; 加拿大自然科学与工程研究理事会;

关键词：

PROTEIN PHOSPHATASE; TARGETING SUBUNIT; GLYCOGEN; GLYCOGEN METABOLISM; CYCLIC AMP-DEPENDENT PROTEIN KINASE;

D O I：

10.1016/0014-5793(95)01184-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A full-length cDNA encoding the putative hepatic glycogen-binding (G(L)) subunit of protein phosphatase-1 (PP1) was isolated from a rat liver library, The deduced amino acid sequence (284 residues, 32.6 kDa) was 23% identical (39% similar) to the N-terminal region of the glycogen-binding (G(M)) subunit of PP1 from striated muscle, The similarities between G(M) and G(L) were most striking between residues 63-86, 144-166 and 186-227 of human G(M) (similar to 40% identity), nearly all the identities with the putative yeast homologue GAC1 being located between 144-166 and 186-227. The cDNA was expressed in E. coli, and the expressed protein transformed the properties of PP1 to those characteristic of the hepatic glycogen-associated enzyme. These experiments establish that the cloned protein is G(L).

引用

页码：294 / 298

页数：5

共 34 条

[1] THE PROTEIN PHOSPHATASES INVOLVED IN CELLULAR-REGULATION - EVIDENCE THAT DEPHOSPHORYLATION OF GLYCOGEN-PHOSPHORYLASE AND GLYCOGEN-SYNTHASE IN THE GLYCOGEN AND MICROSOMAL FRACTIONS OF RAT-LIVER ARE CATALYZED BY THE SAME ENZYME - PROTEIN PHOSPHATASE-1 [J].

ALEMANY, S ;

PELECH, S ;

BRIERLEY, CH ;

COHEN, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 156 (01) :101-110

[2] PHOSPHORYLASE-A IS AN ALLOSTERIC INHIBITOR OF THE GLYCOGEN AND MICROSOMAL FORMS OF RAT HEPATIC PROTEIN PHOSPHATASE-1 [J].

ALEMANY, S ;

COHEN, P .

FEBS LETTERS, 1986, 198 (02) :194-202

[3] THE CONTROL OF PROTEIN PHOSPHATASE-1 BY TARGETING SUBUNITS - THE MAJOR MYOSIN PHOSPHATASE IN AVIAN SMOOTH-MUSCLE IS A NOVEL FORM OF PROTEIN PHOSPHATASE-1 [J].

ALESSI, D ;

MACDOUGALL, LK ;

SOLA, MM ;

IKEBE, M ;

COHEN, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 210 (03) :1023-1035

[4]

ALESSI DR, 1995, METHOD ENZYMOL, V255, P279

[5] THE MODULATOR PROTEIN DISSOCIATES THE CATALYTIC SUBUNIT OF HEPATIC PROTEIN PHOSPHATASE-G FROM GLYCOGEN [J].

BOLLEN, M ;

STALMANS, W .

BIOCHEMICAL JOURNAL, 1988, 250 (03) :659-663

[6] CHARACTERIZATION OF GLYCOGEN-SYNTHASE PHOSPHATASE AND PHOSPHORYLASE-PHOSPHATASE IN SUBCELLULAR LIVER FRACTIONS [J].

BOLLEN, M ;

VANDENHEEDE, JR ;

GORIS, J ;

STALMANS, W .

BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 969 (01) :66-77

[7] MOLECULAR-CLONING OF CDNA-ENCODING THE 110-KDA AND 21-KDA REGULATORY SUBUNITS OF SMOOTH-MUSCLE PROTEIN PHOSPHATASE-1M [J].

CHEN, YH ;

CHEN, MX ;

ALESSI, DR ;

CAMPBELL, DG ;

SHANAHAN, C ;

COHEN, P ;

COHEN, PTW .

FEBS LETTERS, 1994, 356 (01) :51-55

[8] SEQUENCE OF THE HUMAN GLYCOGEN-ASSOCIATED REGULATORY SUBUNIT OF TYPE-1 PROTEIN PHOSPHATASE AND ANALYSIS OF ITS CODING REGION AND MESSENGER-RNA LEVEL IN MUSCLE FROM PATIENTS WITH NIDDM [J].

CHEN, YH ;

HANSEN, L ;

CHEN, MX ;

BJORBAEK, C ;

VESTERGAARD, H ;

HANSEN, T ;

COHEN, PTW ;

PEDERSEN, O .

DIABETES, 1994, 43 (10) :1234-1241

[9]

COHEN P, 1988, METHOD ENZYMOL, V159, P390

[10] IDENTIFICATION OF 3 INVIVO PHOSPHORYLATION SITES ON THE GLYCOGEN-BINDING SUBUNIT OF PROTEIN PHOSPHATASE-1 FROM RABBIT SKELETAL-MUSCLE, AND THEIR RESPONSE TO ADRENALINE [J].

DENT, P ;

CAMPBELL, DG ;

CAUDWELL, FB ;

COHEN, P .

FEBS LETTERS, 1990, 259 (02) :281-285

← 1 2 3 4 →