RECA-DNA HELICAL FILAMENTS IN GENETIC-RECOMBINATION

被引:13
作者
STASIAK, AZ [1 ]
ROSSELLI, W [1 ]
STASIAK, A [1 ]
机构
[1] UNIV LAUSANNE,ANAL ULTRASTRUCT LAB,BATIMENT BIOL,CH-1015 LAUSANNE,SWITZERLAND
关键词
RECA PROTEIN; HOMOLOGOUS RECOMBINATION; DNA STRAND EXCHANGE; ENERGETICS OF RECOMBINATION REACTION; PROTEIN-DNA INTERACTIONS;
D O I
10.1016/0300-9084(91)90203-D
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Paul Howard-Flanders et al proposed a molecular model of RecA-mediated recombination reaction six years ago [1]. How does this model stand at present? In answering this question, we focus on two leading ideas of the original model, namely the proposal of the coaxial arrangement of the aligned DNA molecules within helical RecA filaments and the proposal of the ATP independence of the pairing stage of the recombination reaction. Results obtained after the model was proposed are reviewed and compared with these original assumptions and postulates of the model. EM visualization of recombining DNA molecules, studies of the energetics of the RecA-mediated recombination reaction and biochemical analysis of deproteinized joint molecules are fully consistent with a triple-stranded DNA arrangement during the RecA-mediated recombination reaction and demonstrate the ATP independence of the pairing stage of the reaction.
引用
收藏
页码:199 / 208
页数:10
相关论文
共 31 条
[1]   THE BINDING OF RECA PROTEIN TO DUPLEX DNA-MOLECULES IS DIRECTIONAL AND IS PROMOTED BY A SINGLE STRANDED REGION [J].
CASSUTO, E ;
HOWARDFLANDERS, P .
NUCLEIC ACIDS RESEARCH, 1986, 14 (03) :1149-1157
[2]   VISUALIZATION OF THE PARANEMIC JOINING OF HOMOLOGOUS DNA-MOLECULES CATALYZED BY THE RECA PROTEIN OF ESCHERICHIA-COLI [J].
CHRISTIANSEN, G ;
GRIFFITH, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (07) :2066-2070
[3]  
COX MM, 1982, J BIOL CHEM, V257, P8523
[4]   DIRECTIONALITY AND POLARITY IN RECA PROTEIN-PROMOTED BRANCH MIGRATION [J].
COX, MM ;
LEHMAN, IR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (10) :6018-6022
[5]   THE TOPOLOGY OF HOMOLOGOUS PAIRING PROMOTED BY RECA PROTEIN .6. [J].
DASGUPTA, C ;
SHIBATA, T ;
CUNNINGHAM, RP ;
RADDING, CM .
CELL, 1980, 22 (02) :437-446
[6]   COMPLEXES OF RECA PROTEIN IN SOLUTION - A STUDY BY SMALL-ANGLE NEUTRON-SCATTERING [J].
DICAPUA, E ;
SCHNARR, M ;
RUIGROK, RWH ;
LINDNER, P ;
TIMMINS, PA .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 214 (02) :557-570
[7]   CHARACTERIZATION OF COMPLEXES BETWEEN RECA PROTEIN AND DUPLEX DNA BY ELECTRON-MICROSCOPY [J].
DICAPUA, E ;
ENGEL, A ;
STASIAK, A ;
KOLLER, T .
JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) :87-103
[8]   ISOLATION AND VISUALIZATION OF ACTIVE PRESYNAPTIC FILAMENTS OF RECA PROTEIN AND SINGLE-STRANDED-DNA [J].
FLORY, J ;
TSANG, SS ;
MUNIYAPPA, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (22) :7026-7030
[9]  
HINIGBERG SM, 1985, J BIOL CHEM, V260, P11845
[10]   ROLE OF RECA PROTEIN SPIRAL FILAMENTS IN GENETIC-RECOMBINATION [J].
HOWARDFLANDERS, P ;
WEST, SC ;
STASIAK, A .
NATURE, 1984, 309 (5965) :215-220