BLEOMYCIN BINDING-SITES ON ALVEOLAR MACROPHAGES

被引:11
作者
DENHOLM, EM [1 ]
PHAN, SH [1 ]
机构
[1] UNIV MICHIGAN,DEPT PATHOL,ANN ARBOR,MI 48109
关键词
fibroblast growth factors; lung; monocyte chemotactic factors; pulmonary fibrosis;
D O I
10.1002/jlb.48.6.519
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Previous work has demonstrated that bleomycin can directly stimulate alveolar macrophage secretion of fibroblast growth factors and monocyte chemotactic factors. In this study, rat alveolar macrophages obtained by bronchoalveolar lavage were examined for the presence of bleomycin binding sites, which might mediate this response. The results indicated that alveolar macrophages have specific, saturable, and reversible binding sites. Both high- and low-affinity binding sites were found; each macrophage possessed 6.7 x 104 high-affinity sites, with a K(d) of 528 nM, and 2.2 x 106 low-affinity sites, with a K(d) of 65 μM. The K(d) of the high-affinity sites corresponds closely to the ED50 obtained from dose-response curves of the bleomycin-stimulated secretion of both fibroblast growth and monocyte chemotactic factors, suggesting that bleomycin stimulation of alveolar macrophage function responses may be mediated by bleomycin interaction with these sites.
引用
收藏
页码:519 / 523
页数:5
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