PURIFICATION AND SOME PROPERTIES OF AN ATYPICAL ALKALINE PARA-NITROPHENYLPHOSPHATE PHOSPHATASE-ACTIVITY FROM HALOBACTERIUM-HALOBIUM

被引：14

作者：

BONET, ML ^{[1
]}

LLORCA, FI ^{[1
]}

CADENAS, E ^{[1
]}

机构：

[1] UNIV ALICANTE,FAC CIENCIAS,DIV BIOQUIM,APTDO 99,E-03080 ALICANTE,SPAIN

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1991年 / 23卷 / 12期

关键词：

D O I：

10.1016/0020-711X(91)90288-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. An alkaline p-nitrophenylphosphate phosphatase has been purified 440-fold from extracts of Halobacterium halobium. 2. The enzyme has an apparent molecular weight of 24,000. 3. A K(m) value for p-nitrophenylphosphate of 1.12 mM has been found under optimal conditions. 4. The enzyme is selectively activated and stabilized by Mn2+. 5. It requires high salt concentrations for stability and maximum activity. 6. It displays an unusual restricted substrate specificity of 25 phosphate esters tested, only phosphotyrosine and casein were hydrolysed besides p-nitrophenylphosphate.

引用

页码：1445 / 1451

页数：7

共 36 条

[1] A NEW GLUTAMATE-DEHYDROGENASE FROM HALOBACTERIUM-HALOBIUM WITH DIFFERENT COENZYME SPECIFICITY [J].

BONETE, MJ ;

CAMACHO, ML ;

CADENAS, E .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1987, 19 (12) :1149-1155

[2]

CATHALA G, 1975, J BIOL CHEM, V250, P6046

[3]

COLEMAN JE, 1983, ADV ENZYMOL RAMB, V55, P381

[4]

CYBORON GW, 1981, J BIOL CHEM, V256, P7272

[5] PURIFICATION AND PROPERTIES OF PYRUVATE-KINASE FROM HALOBACTERIUM-CUTIRUBRUM [J].

DEMEDICIS, E ;

LALIBERTE, JF ;

VASSMARENGO, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 708 (01) :57-67

[6]

FARLEY JR, 1980, J BIOL CHEM, V255, P4680

[7] ISOLATION AND PROPERTIES OF A SMALL MANGANESE-ION-STIMULATED BACTERIAL ALKALINE-PHOSPHATASE [J].

FITT, PS ;

PETERKIN, PI .

BIOCHEMICAL JOURNAL, 1976, 157 (01) :161-167

[8]

GLEW RH, 1971, J BIOL CHEM, V246, P1556

[9] CATALYTIC PROPERTIES OF ALKALINE-PHOSPHATASE FROM PIG KIDNEY [J].

HIWADA, K ;

WACHSMUTH, ED .

BIOCHEMICAL JOURNAL, 1974, 141 (01) :283-291

[10] INACTIVATION AND REACTIVATION OF SALT-REQUIRING ENZYMES FROM AN EXTREME OBLIGATE HALOPHILE [J].

HOLMES, PK ;

HALVORSON, HO .

CANADIAN JOURNAL OF MICROBIOLOGY, 1963, 9 (06) :904-&

← 1 2 3 4 →