EFFECT OF PHOSPHORAMIDON ON BIG ENDOTHELIN-2 CONVERSION INTO ENDOTHELIN-2 IN HUMAN RENAL ADENOCARCINOMA (ACHN) CELLS - ANALYSIS OF ENDOTHELIN-2 BIOSYNTHETIC-PATHWAY

被引：12

作者：

YORIMITSU, K

SHINMI, O

NISHIYAMA, M

MOROI, K

SUGITA, Y

SAITO, T

INAGAKI, Y

MASAKI, T

KIMURA, S

机构：

[1] CHIBA UNIV,SCH MED,CTR NEUROBIOL & MOLEC IMMUNOL,CHIBA 260,JAPAN

[2] CHIBA UNIV,DEPT INTERNAL MED 3,CHIBA 260,JAPAN

[3] TSUKUBA UNIV,INST BASIC MED SCI,DEPT BIOCHEM,TSUKUBA 305,JAPAN

[4] KYOTO UNIV,SCH MED,DEPT PHARMACOL,KYOTO 606,JAPAN

来源：

FEBS LETTERS | 1992年 / 314卷 / 03期

关键词：

ENDOTHELIN-2; BIG ENDOTHELIN-2; ENDOTHELIN-CONVERTING ENZYME; PHOSPHORAMIDON; HUMAN RENAL ADENOCARCINOMA (ACHN) CELL;

D O I：

10.1016/0014-5793(92)81513-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The biosynthetic pathway of endothelin (ET)-2 was analyzed in cultured ACHN cells. In the supernatant, we detected three ET-2-related peptides, ET-2, big ET-2(1-38) and big ET-2(22-38). Phosphoramidon decreased the amount of ET-2 and increased that of big ET-2(1-38) dose-dependently. The amount of big ET-2(1-37) did not significantly change. These results suggest that big ET-2 is composed of 38 and not 37 amino acid residues, and that a putative ET-2-converting enzyme (ECE-2) should be classified as a phosphoramidon-sensitive neutral metalloprotease, bearing a resemblance to the putative ET-1-converting enzyme (ECE-1) in endothelial cells.

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收藏

页码：395 / 398

页数：4

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