A SHORT INTERSPERSED REPETITIVE ELEMENT PROVIDES A NEW 3' ACCEPTOR SITE FOR TRANSSPLICING IN CERTAIN RIBOSOMAL P2-BETA PROTEIN GENES OF TRYPANOSOMA-CRUZI

被引：35

作者：

VAZQUEZ, MP ^{[1
]}

SCHIJMAN, AG ^{[1
]}

LEVIN, MJ ^{[1
]}

机构：

[1] INST INVEST INGN GENET & BIOL MOLEC,RA-1428 BUENOS AIRES,DF,ARGENTINA

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1994年 / 64卷 / 02期

关键词：

TRYPANOSOMA CRUZI; RIBOSOMAL PROTEIN; REPETITIVE SEQUENCE; TRANSSPLICING; SPLICED LEADER;

D O I：

10.1016/0166-6851(94)00026-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Four Trypanosoma cruzi genomic DNA fragments carrying different TcP2 beta genes have been isolated and sequenced. Three of them had a single TcP2 beta gene, while the 3.8-kb-long DNA segment encoding the TcP2 beta-H1.8 locus showed two TcP2 beta genes arranged in tandem. These genes were physically connected by a 428-bp-long DNA sequence that was also located immediately 5' to the first gene and immediately 3' to the second. Comparison of the 4 TcP2 beta gene loci, suggested that the insertion of this repeated element originated the duplication of its target sequence, a poly(dT) stretch. Approximately 1200 copies of this short sequence, named short interspersed repetitive element (SIRE), were found scattered in the genome. Analysis of the 5' non-coding regions of different TcP2 beta mRNAs, and RNA-PCR experiments suggested that the insertion of a SIRE upstream of a TcP2 beta-H1.8 gene introduced a new 3' spliced leader (SL) acceptor site in the TcP2 beta-H1.8 pre-mRNAs, encoded within the SIRE. Consequently, in the mature H1.8 mRNA the SL sequence is followed by 38 bases directly transcribed from the SIRE. Structural and functional features of this repeated element reveal similarity to the short interspersed repetitive DNA sequences detected in the genomes of several microorganisms.

引用

页码：327 / 336

页数：10

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