DEVELOPMENT OF MULTI-RESIDUE METHODOLOGY FOR THE HPLC DETERMINATION OF VETERINARY DRUGS IN ANIMAL-TISSUES

被引：21

作者：

COOPER, AD

CREASER, CS

FARRINGTON, WHH

TARBIN, JA

SHEARER, G

机构：

[1] Ministry of Agriculture, Fisheries and Food, Food Science Laboratory, Norwich Research Park, Colney, Norwich

[2] Department of Chemistry and Physics, Nottingham Trent University, Nottingham, NG11 8NS, Clifton Lane

来源：

FOOD ADDITIVES AND CONTAMINANTS | 1995年 / 12卷 / 02期

关键词：

MULTI-RESIDUE; VETERINARY DRUGS; SOLID-PHASE EXTRACTION; HPLC; UV-DIODE ARRAY DETECTION;

D O I：

10.1080/02652039509374291

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The application of solid-phase extraction and HPLC with UV-diode array detection to the multi-residue determination of veterinary drugs is described. A two-stage SPE clean-up was employed, using C18 and silica cartridges. HPLC analysis was carried out on a base-deactivated C8 column using gradient elution systems at two pH values. The procedure establishes the basis of a method for routine screening of pig kidney samples for some sulphonamides, benzimidazoles, nitroimidazoles and nitrofurans at concentrations at or below the UK Maximum Residue Limits (MRLs). Limits of detection of 2-18 mu g/kg could be achieved for these analytes at recoveries of 40-70%. UV spectra measured on-line were used for confirmation of peak identities at these concentrations. The possibility of extension of this procedure to a wider range of analytes is discussed.

引用

页码：167 / 176

页数：10

共 13 条

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