SPECIFIC BINDING OF [H-3] HEPARIN TO HUMAN CARCINOMA SW-13 AND OTHER MAMMALIAN-CELLS

被引：11

作者：

HALPER, J

机构：

[1] Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens

来源：

EXPERIMENTAL CELL RESEARCH | 1990年 / 187卷 / 02期

关键词：

D O I：

10.1016/0014-4827(90)90099-V

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

This study reports on the specific binding of [3H]heparin to human adrenocortical carcinoma cell line SW-13. Heparin binding to SW-13 cells is specific, saturable, and time- and temperature-dependent with maximum binding occurring between 90 and 120 min at 22 °C. Scatchard analysis revealed two classes of binding sites. The apparent Kd for high-affinity receptors is 2.14 × 10-8 M with 1.48 × 106 sites per cells. Six other tested mammalian cell lines also have specific binding sites for heparin. © 1990.

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页码：324 / 327

页数：4

相关论文

共 27 条

[1] DEVELOPMENT OF 3T3-LIKE LINES FROM BALB/C MOUSE EMBRYO CULTURES - TRANSFORMATION SUSCEPTIBILITY TO SV40 [J].

AARONSON, SA ;

TODARO, GJ .

JOURNAL OF CELLULAR PHYSIOLOGY, 1968, 72 (2P1) :141-&

[2] HEPARIN AND HEPARAN-SULFATE BINDING-SITES ON B-16 MELANOMA-CELLS [J].

BISWAS, C .

JOURNAL OF CELLULAR PHYSIOLOGY, 1988, 136 (01) :147-153

[3] BINDING AND INTERNALIZATION OF HEPARIN BY VASCULAR SMOOTH-MUSCLE CELLS [J].

CASTELLOT, JJ ;

WONG, K ;

HERMAN, B ;

HOOVER, RL ;

ALBERTINI, DF ;

WRIGHT, TC ;

CALEB, BL ;

KARNOVSKY, MJ .

JOURNAL OF CELLULAR PHYSIOLOGY, 1985, 124 (01) :13-20

[4] INSITU DETECTION OF MYCOPLASMA CONTAMINATION IN CELL-CULTURES BY FLUORESCENT HOECHST-33258 STAIN [J].

CHEN, TR .

EXPERIMENTAL CELL RESEARCH, 1977, 104 (02) :255-262

[5] SUPPRESSION BY HEPARIN OF SMOOTH-MUSCLE CELL-PROLIFERATION IN INJURED ARTERIES [J].

CLOWES, AW ;

KARNOWSKY, MJ .

NATURE, 1977, 265 (5595) :625-626

[6]

COFFEY AK, 1985, AM J PATHOL, V120, P248

[7]

GIERD DJ, 1973, J NATL CANCER I, V51, P1417

[8] EFFECT OF FIBROBLAST GROWTH-FACTOR AND LIPOPROTEINS ON THE PROLIFERATION OF ENDOTHELIAL-CELLS DERIVED FROM BOVINE ADRENAL-CORTEX, BRAIN CORTEX, AND CORPUS-LUTEUM CAPILLARIES [J].

GOSPODAROWICZ, D ;

MASSOGLIA, S ;

CHENG, J ;

FUJII, DK .

JOURNAL OF CELLULAR PHYSIOLOGY, 1986, 127 (01) :121-136

[9] INHIBITION OF RAT ARTERIAL SMOOTH-MUSCLE CELL-PROLIFERATION BY HEPARIN - INVIVO STUDIES WITH ANTI-COAGULANT AND NON-ANTICOAGULANT HEPARIN [J].

GUYTON, JR ;

ROSENBERG, RD ;

CLOWES, AW ;

KARNOVSKY, MJ .

CIRCULATION RESEARCH, 1980, 46 (05) :625-634

[10] MODULATION OF GROWTH OF HUMAN CARCINOMA SW-13 CELLS BY HEPARIN AND GROWTH-FACTORS [J].

HALPER, J ;

CARTER, BJ .

JOURNAL OF CELLULAR PHYSIOLOGY, 1989, 141 (01) :16-23