CHARACTERIZATION OF PRIMARY HUMAN FIBROBLASTS TRANSFORMED BY HUMAN PAPILLOMA-VIRUS TYPE-16 AND HERPES-SIMPLEX VIRUS TYPE-2 DNA-SEQUENCES

被引:17
作者
DHANWADA, KR
VEERISETTY, V
ZHU, F
RAZZAQUE, A
THOMPSON, KD
JONES, C
机构
[1] UNIV NEBRASKA,CTR BIOTECHNOL,DEPT VET SCI,LINCOLN,NE 68583
[2] LOYOLA UNIV,CHICAGO MED CTR,DEPT MICROBIOL,MAYWOOD,IL 60153
[3] UNIV MISSISSIPPI,MED CTR,DEPT MICROBIOL,JACKSON,MS 39216
[4] US FDA,DIV VIROL,BETHESDA,MD 20205
关键词
D O I
10.1099/0022-1317-73-4-791
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Human papilloma virus type 16 (HPV-16) and herpes simplex virus type 2 (HSV-2) are human viruses implicated in the development of cancer, in particular cervical cancer. The ability of HSV-2 and HPV-16 to transform early passage human cells was examined in this report. For these studies, gingival fibroblasts were utilized. One gingival cell strain was derived from a normal individual (N-16). The second cell strain was derived from hyperplastic gingival tissue of an epileptic individual (R-30) treated with phenytoin, an antiseizure drug. A common side effect of phenytoin is the induction of gingival overgrowth. R-30 cells contained a stable chromosomal translocation between chromosomes 8 and 18 and expressed higher steady state levels of c-myc. HPV-16 DNA efficiently immortalized R-30 cells but not N-16 cells. R-30 cells cotransfected with HPV-16, and HSV-2 viral DNAs were more aneuploid than R-30 cells transfected with HPV-16 DNA alone. Additionally, R-30 cells cotransfected with both viral DNAs grew better in soft agar than R-30 cells transfected with HPV-16 DNA alone. HSV-2 DNA was detected in transformed cells by polymerase chain reaction. These results suggested R-30 cells were immortalized more efficiently by HPV-16 and further imply that HPV-16 and HSV-2 DNA fragments can cooperate during multistep transformation.
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页码:791 / 799
页数:9
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