INTRACELLULAR GLUTATHIONE AND ITS METABOLIZING ENZYME-ACTIVITIES IN A METASTATIC VARIANT MELANOMA CELL-LINE

被引：12

作者：

CHAKRABORTY, AK

UEDA, M

MISHIMA, Y

ICHIHASHI, M

机构：

[1] Department of Dermatology, Yale University School of Medicine, New Haven, CT, 06510

[2] Department of Dermatology, Kobe University School of Medicine, Kobe, 650, 5-1 Kusunoki Cho 7-Chome, CHUO-KU

[3] Mishima Institute for Dermatological Research, Kobe, 650, 17-8-801, 3-Chome, Motomachi-dori, CHUO-KU

来源：

MELANOMA RESEARCH | 1992年 / 2卷 / 5-6期

关键词：

5-S-CYSTEINYLDOPA; ENZYMES; GLUTATHIONE; MELANOMA; METASTASIS;

D O I：

10.1097/00008390-199212000-00004

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Levels of glutathione peroxidase (GSH-PX), glutathione-S-transferase (GST), gamma-glutamyl transpeptidase (gammaGTP) and glutathione reductase (GR) activities in the B16-F10 metastatic melanoma cell line are higher than those in non-metastatic B-16 murine melanoma cells. An inverse relationship was observed between the level of reduced glutathione (GSH) and metastatic capacity. Interferon (IFN), an antitumour and antimetastic agent, reduced the experimental metastatic capacity of B16-F10 cells while increasing the intracellular GSH content. This was associated with a fall in activity of GSH-metabolizing enzymes. These results suggest a correlation of intracellular GSH and its metabolizing enzymes with malignant transformation.

引用

页码：315 / 319

页数：5

共 50 条

[1]

Prota G., Recent advances in the chemistry of pheomelano-genesis in mammals, J Invest Dermatol, 75, pp. 122-127, (1980)

[2]

Agrup G., Agrup P., Ersson T., Et al., Urinary excretion of 5SCD in patients with primary melanoma or melanoma metastasis, Acta Dermatol Venereol (Stockh), 55, pp. 337-341, (1975)

[3]

Agrup G., Falck B., Kennedy B., Et al., Formation of cysteinyldopa from glutathioyldopa in melanoma, Acta Dermatol Venereol (Stockh), 55, pp. 1-3, (1975)

[4]

Agrup G., Agrup P., Ersson T., Et al., Five years’ experience of 5-S-cysteinyldopa in melanoma diagnosis, Acta Dermatol Venereol (Stockh), 59, pp. 381-388, (1979)

[5]

Mishima Y., Ichihashi M., Mojamder M., Production, excretion and regulatory factors of 5SCD genesis in melanoma cells: Implications for mixed Eu- and Pheo melanogenesis, Pigment Cell, pp. 709-716, (1985)

[6]

Rorsman H., Agrup G., Falck B., Et al., Exposure to sunlight and urinary excretion of 5-S-cysteinyl dopa, Pigment Cell, 2, pp. 284-289, (1976)

[7]

Chakraborty A.K., Ichihashi M., Mishima Y., Effect of dopa loading on glutathione metabolizing enzymes and tyrosinase in relation to 5SCD genesis in cultured B-16 melanoma cells, J Dermatol Sci, 2, pp. 329-335, (1991)

[8]

Mojamder M., Ichihashi M., Mishima Y., γ-Glutamyl transpeptidase, tyrosinase and 5 S-cysteinyldopa production in melanoma cells, J Invest Dermatol, 81, pp. 119-121, (1983)

[9]

Hu F., Theophylline and melanocyte stimulating hormone effects on γ-GTP and dopa reaction in cultured melanoma cells, J Invest Dermatol, 79, pp. 57-61, (1982)

[10]

Benedetto J., Ortonne J., Voulet C., Et al., Role of thiol compounds in mammalian melanin pigmentation. II Glutathione and related enzymatic activities, J Invest Dermatol, 79, pp. 422-424, (1982)

← 1 2 3 4 5 →