RESOLUTION OF CARBOXYLIC-ACID ENANTIOMERS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH HIGHLY SENSITIVE LASER-INDUCED FLUORESCENCE DETECTION

Optical resolution of carboxylic acid enantiomers by high-performance liquid chromatography with laser-induced fluorescence detection after chiral derivatization was investigated. Both laser power and time constants influence the detection limit of the fluorophore. The minimum detectable levels of three derivatives of naproxen [4-(aminosulphonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzodioxazole, 4-(N,N-dimethylaminosulphonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole and 4-nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzodiazole derivatives] were in the femtomole-attomole ranges, and the lowest detection limit was obtained with the last derivative.