A NOVEL 6FE (2X[3FE-4S]) FERREDOXIN FROM MYCOBACTERIUM-SMEGMATIS

被引：8

作者：

IMAI, T ^{[1
]}

URUSHIYAMA, A ^{[1
]}

SAITO, H ^{[1
]}

SAKAMOTO, Y ^{[1
]}

OTA, K ^{[1
]}

OHMORI, D ^{[1
]}

机构：

[1] JUNTENDO UNIV, DEPT CHEM, INBA, CHIBA 27016, JAPAN

来源：

FEBS LETTERS | 1995年 / 368卷 / 01期

关键词：

6FE FERREDOXIN; MYCOBACTERIUM SMEGMATIS; 3FE-4S; RAMAN;

D O I：

10.1016/0014-5793(95)00601-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel ferredoxin was purified from Mycobacterium smegmatis by a series of hydrophobic chromatographies in the presence of high concentrations of ammonium sulfate and sodium chloride, The ferredoxin exhibited the same peptide map and N-terminal amino acid sequence as the known 7Fe ferredoxin from the same bacterium, On the other hand, this ferredoxin was found to contain similar to 6 Fe/mol ferredoxin and was also shown to contain only [3Fe-4S] clusters by resonance Raman spectroscopy, indicating that it is a novel 6Fe ferredoxin which contains two [3Fe-4S] clusters.

引用

页码：23 / 26

页数：4

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