Principle and clinical usefulness of the infrared fluorescence endoscopy

被引：16

作者：

Ito, Susumu ^{[1
]}

Muguruma, Naoki

Kimura, Tetsuo

Yano, Hiromi

Imoto, Yoshitaka

Okamoto, Koichi

Kaji, Masako

Sano, Shigeki

Nagao, Yoshimitsu

机构：

[1] Univ Tokushima, Grad Sch, Inst Hlth Biosci, Dept Digest & Cardiovasc Med, Tokushima, Japan

来源：

JOURNAL OF MEDICAL INVESTIGATION | 2006年 / 53卷 / 1-2期

关键词：

infrared fluorescence; infrared fluorescence endoscopy; indocyanin green delivative; endoscopic diagnosis; bioendscopy;

D O I：

10.2152/jmi.53.1

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Since there isno infrared fluorescence materials in the living body, infrared fluorescence labeling materials are very useful formaking a diagnosis of a micro cancer. We have developed an infrared fluorescence endoscope (IRFE) and indocyanin green (ICG)-derivative as infrared fluorescence labeling materials to evaluate gastrointestinal neoplastic lesions. The study aims were to apply an IRFE and to demonstrate its usefulness in detecting cancerous tissue using an antibody coupled with ICG-derivative. IRFE consisted of an infrared endoscope equipped with excitation (710-790nm) and barrier (810-920nm) filters and an intensified CCD camera. We have developed ICG N-hydroxy sulfo succinimide ester (ICG-sulfo-OSu) and 3-ICG-acyl-1, 3-thiazolidine-2-thione (ICG-ATT) as an infrared fluorescent-labeling reagent. ICG-derivative-labeledmouseanti-human carcinoembryonic antigen (CEA) antibody and MUC1 antibody were employed in this study. Moreover, we examined the ability of a reinforcement agent, octylglucoside, to intensity fluorescence from the labeled antibody. Biopsy specimens of gastric cancer were stained with anti-CEA antibody by the avidin-biotinylated peroxidase complex method. Among the positive specimens, freshly resected stomach from three cases were used for the infrared (IR) imaging analysis. The incubation of freshly resected stomach specimens with ICG-anti-CEA antibody-complex resulted in positive staining of the tumor sites by IRFE, and the IR fluorescent images correlated well with the tumor sites. The immunohistochemical studies suggested that the intensity of IR fluorescence of ICG-ATT-MUC1was stronger than that of ICG-sulfo-OSu. In tumorsections, the reinforcement agent intensified fluorescence, ever at low antibody concentrations. Therefore, we conclude that an anti-CEA (and/orMUC1) antibody with affinity for cancerous lesions and labeled with ICG-derivative can be imaged with this IRFE. Specific antibodies tagged with ICG-derivative with the reinforcement agent canlabel cancer cells and generate a strong enough fluorescent signal to detect small cancerswhen examined with an IR fluorescence endoscope.

引用

页码：1 / 8

页数：8

共 14 条

[1]

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