A SANDWICH ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR HUMAN INTERLEUKIN-5

被引：33

作者：

FUKUDA, Y ^{[1
]}

HASHINO, J ^{[1
]}

HARUYAMA, M ^{[1
]}

TSURUOKA, N ^{[1
]}

NAKAZATO, H ^{[1
]}

NAKANISHI, T ^{[1
]}

机构：

[1] SUNTORY INST BIOMED RES,1-1-1 WAKAYAMADAI,SHIMAMOTO CHO,OSAKA 618,JAPAN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 143卷 / 01期

关键词：

INTERLEUKIN-5; ELISA; SANDWICH; MONOCLONAL ANTIBODY;

D O I：

10.1016/0022-1759(91)90276-L

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for human interleukin-5 (hIL-5) using a combination of monoclonal anti-recombinant(r)-hIL-5 antibody and rabbit anti-r-hIL-5 IgG. Detection limit of this assay was estimated to be 7.8 pg/ml, which was about 10,000 times more sensitive than that of the bioassay using BCL1 cells of murine origin. This ELISA was specific for hIL-5, showing no crossreactivity to recombinant human GM-CSF, IL-4, TNF-alpha, IFN-gamma and mouse IL-5 (mIL-5). The presence of 10% fetal calf serum did not interfere with the measurement of r-hIL-5. Coefficients of variation in intra-assay and interassay were 1.1-4.6% and 2.3-11.3%, respectively. These results indicate that this assay system can be quite useful in quantifying hIL-5 in various biological fluids.

引用

页码：89 / 94

页数：6

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