MOLECULAR-CLONING OF THE FULL-LENGTH CDNA-ENCODING THE HUMAN CALBINDIN-D9K

被引：36

作者：

JEUNG, EB ^{[1
]}

KRISINGER, J ^{[1
]}

DANN, JL ^{[1
]}

LEUNG, PCK ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA,DEPT OBSTET & GYNAECOL,RES CTR,178-950 W 28TH AVE,VANCOUVER V5Z 4H4,BC,CANADA

来源：

FEBS LETTERS | 1992年 / 307卷 / 02期

关键词：

CALBINDIN; VITAMIN-D; CLONING; PCR;

D O I：

10.1016/0014-5793(92)80772-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The full-length cDNA encoding the human calbindin-D9k (CaBP-9k) has been cloned using reverse transcription/PCR methodology with rat- and bovine-derived primers and intestinal RNA. A core product, and both a 5' and 3' product encompassing the full-length cDNA were obtained. The clones include coding region for 79 amino acids, 57 nucleotides 5'- and 159 nucleotides 3'-non-coding region, and a poly(A) tail. The deduced protein sequence is homologous to other mammalian CaBPs. Northern analysis revealed the mRNA in human duodenum to be about 600 nucleotides in length. Expression levels in adult human tissue were substantially lower than in child, rat or porcine intestine.

引用

页码：224 / 228

页数：5

共 26 条

[1] VITAMIN D-DEPENDENT CALCIUM-BINDING PROTEINS (CABPS) IN HUMAN FETUSES - COMPARATIVE DISTRIBUTION OF 9K-CABP MESSENGER-RNA AND 28K-CABP DURING DEVELOPMENT [J].