CLONING OF MURINE AND RAT VASCULAR CELL-ADHESION MOLECULE-1

被引：109

作者：

HESSION, C ^{[1
]}

MOY, P ^{[1
]}

TIZARD, R ^{[1
]}

CHISHOLM, P ^{[1
]}

WILLIAMS, C ^{[1
]}

WYSK, M ^{[1
]}

BURKLY, L ^{[1
]}

MIYAKE, K ^{[1
]}

KINCADE, P ^{[1
]}

LOBB, R ^{[1
]}

机构：

[1] OKLAHOMA MED RES FDN,OKLAHOMA CITY,OK 73104

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 183卷 / 01期

关键词：

D O I：

10.1016/0006-291X(92)91623-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vascular cell adhesion molecule-1 (VCAM1) is a member of the immunoglobulin (Ig) superfamily which interacts with the integrin very late antigen 4 (VLA4). We have cloned the cDNAs for both murine and rat VCAM1 from endotoxin-treated lung libraries. Both sequences encode proteins with seven extracellular Ig-like domains, which show 75.9% and 76.9% identity, respectively, with human VCAM1. Both murine and human cell lines show VLA4-dependent binding to COS cells transiently expressing murine and rat VCAM1. Two mAbs, M-K 1 and M-K 2, which recognize an antigen on murine bone marrow stromal cell lines, bind to murine VCAM1 expressed in COS cells and block VCAM1-dependent adhesion, confirming that these mAbs recognize murine VCAM1. © 1992.

引用

页码：163 / 169

页数：7

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