DETECTION OF GENE-DISRUPTION EVENTS IN ASPERGILLUS TRANSFORMANTS BY POLYMERASE CHAIN-REACTION DIRECT FROM CONIDIOSPORES

被引：25

作者：

AUFAUVREBROWN, A ^{[1
]}

TANG, CM ^{[1
]}

HOLDEN, DW ^{[1
]}

机构：

[1] HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT INFECT DIS & BACTERIOL, DU CANE RD, LONDON W12 0NN, ENGLAND

来源：

CURRENT GENETICS | 1993年 / 24卷 / 1-2期

关键词：

GENE DISRUPTION; ASPERGILLUS; POLYMERASE CHAIN REACTION;

D O I：

10.1007/BF00324683

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We describe a rapid method for the identification of gene disruption events after DNA-mediated transformation of Aspergillus fumigatus. This involves a polymerase chain reaction in which the target DNA is added in the form of intact conidiospores. Using one primer specific to the plasmid DNA and a second primer specific to the target gene on the chromosome, it is possible to identify gene disruption events among the more common ectopic integrations approximately 4 h after sporulating transformants appear on selective medium.

引用

页码：177 / 178

页数：2

共 6 条

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