SPECTROPHOTOMETRIC STUDY OF THE INTERACTION OF TETRAPHENYLPORPHYRIN TETRASULFONATE (TPPS4) WITH PROTEINS

被引：21

作者：

LI, N

TONG, SY

机构：

[1] Chemistry Department, Peking University, Beijing

来源：

TALANTA | 1994年 / 41卷 / 10期

关键词：

D O I：

10.1016/0039-9140(94)E0096-A

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The interaction of water-soluble porphyrin TPPS4 (tetraphenylporphyrin tetrasulfonate) with proteins in acidic solution was studied by spectrophotometry. The absorption spectra of TPPS4-protein complexes, the aggregation of TPPS4 in acidic solution, and comparison of the absorption spectra of TPPS4-protein conjugate with that of the TPPS4-protein complex was investigated in detail. The effects of denaturants including urea and SDS were also examined. A mechanism was proposed that TPPS4 would be distributed between microphase of protein and the aqueous solution and then aggregated in the microphase.

引用

页码：1657 / 1662

页数：6

共 9 条

[1]

DOLPHIN D, 1978, PORPHYRINS, V5, P307

[2] ALPHA,BETA,GAMMA,DELTA-TETRA-(4-PYRIDYL)-PORPHINE AND SOME OF ITS METAL COMPLEXES [J].