PREPARATIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC PURIFICATION OF SAFFRON SECONDARY METABOLITES

被引：41

作者：

CASTELLAR, MR ^{[1
]}

MONTIJANO, H ^{[1
]}

MANJON, A ^{[1
]}

IBORRA, JL ^{[1
]}

机构：

[1] UNIV MURCIA,FAC CHEM,DEPT BIOCHEM & MOLEC BIOL B & IMMUNOL,POB 4021,E-30001 MURCIA,SPAIN

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 648卷 / 01期

关键词：

D O I：

10.1016/0021-9673(93)83301-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A preparative HPLC procedure to isolate picrocrocin, the compound responsible for the taste of saffron and precursor of the aromatic safranal, and the mixture of yellow pigments from a saffron hydroalcoholic extract has been developed. A reversed-phase C18 column was employed as the stationary phase. The best separation was obtained with 45% methanol, plus a 90% acetonitrile pulse 3 min after starting the run, as mobile phase. To obtain the highest yield from the system, sample size was increased up to 2 ml of 200 mg ml-1 saffron extract; under such conditions a good resolution was obtained and picrocrocin and saffron pigments were separated with a high purification yield and purity.

引用

页码：187 / 190

页数：4

共 15 条

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