FORMATION OF A CYTOSOLIC GANGLIOSIDE PROTEIN COMPLEX FOLLOWING ADMINISTRATION OF PHOTOREACTIVE GANGLIOSIDE GM1 TO HUMAN FIBROBLASTS IN CULTURE

被引：36

作者：

CHIGORNO, V ^{[1
]}

VALSECCHI, M ^{[1
]}

ACQUOTTI, D ^{[1
]}

SONNINO, S ^{[1
]}

TETTAMANTI, G ^{[1
]}

机构：

[1] UNIV MILAN,SCH MED,DEPT MED CHEM & BIOCHEM,STUDY CTR FUNCT BIOCHEM BRAIN LIPID,I-20133 MILAN,ITALY

来源：

FEBS LETTERS | 1990年 / 263卷 / 02期

关键词：

Cytosolic complex; Ganglioside-protein interaction; Photoreactive GM1;

D O I：

10.1016/0014-5793(90)81406-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A GM1 ganglioside derivative bearing a photoreactive nitrophenyl azide group and tritium labeled at the acetyl group of N-acetylneuraminic acid, has been administered to cultured human fibroblasts. With photolabeling experiments we found that a portion of the ganglioside in the cell cytosol was associated with a soluble protein of about 30 kDa. © 1990.

引用

页码：329 / 331

页数：3

共 20 条

[1] CHIGORNO V, 1986, ANAL BIOCHEM, V153, P283, DOI 10.1016/0003-2697(86)90094-1
[2] Chigorno V., 1985, GLYCOCONJUGATE J, V2, P279
[3] PROMOTION OF NEURITOGENESIS IN MOUSE NEURO-BLASTOMA CELLS BY EXOGENOUS GANGLIOSIDES - RELATIONSHIP BETWEEN THE EFFECT AND THE CELL ASSOCIATION OF GANGLIOSIDE GM1
FACCI, L
LEON, A
TOFFANO, G
SONNINO, S
GHIDONI, R
TETTAMANTI, G
[J]. JOURNAL OF NEUROCHEMISTRY, 1984, 42 (02) : 299 - 305
[4] GHIDONI R, 1980, J BIOL CHEM, V255, P6990
[5] INCORPORATION OF A GANGLIOSIDE AND A SPIN-LABELED GANGLIOSIDE ANALOG INTO CELL AND LIPOSOMAL MEMBRANES
KANDA, S
INOUE, K
NOJIMA, S
UTSUMI, H
WIEGANDT, H
[J]. JOURNAL OF BIOCHEMISTRY, 1982, 91 (06) : 2095 - 2098
[6] Ledeen R W, 1976, Adv Exp Med Biol, V71, P83
[7] LEDEEN RW, 1987, NATO ASI SERIES H, V7, P259
[8] LOWRY OH, 1951, J BIOL CHEM, V193, P265
[9] USE OF BUTYL ACETATE IN DETERMINATION OF SIALIC ACID
MIETTINEN, T
TAKKILUUKKAINEN, IT
[J]. ACTA CHEMICA SCANDINAVICA, 1959, 13 (04): : 856 - 858
[10] SOLUBLE GANGLIOSIDES IN CULTURED NEUROTUMOR CELLS
MILLERPODRAZA, H
FISHMAN, PH
[J]. JOURNAL OF NEUROCHEMISTRY, 1983, 41 (03) : 860 - 867

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