STABLE TRANSFORMATION OF BARLEY VIA PEG-INDUCED DIRECT DNA UPTAKE INTO PROTOPLASTS

被引：105

作者：

LAZZERI, PA

BRETTSCHNEIDER, R

LUHRS, R

LORZ, H

机构：

[1] Institute for General Botany (AMP II), University of Hamburg, Hamburg, 52

来源：

THEORETICAL AND APPLIED GENETICS | 1991年 / 81卷 / 04期

关键词：

HORDEUM-VULGARE; PROTOPLASTS; DNA UPTAKE; STABLE TRANSFORMATION; CEREALS;

D O I：

10.1007/BF00219433

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Protoplasts isolated from a barley cell suspension (cv Dissa) were transformed with plasmid DNA containing the neomycinphosphotransferase II (NPT) and beta-glucuronidase (GUS) genes, using polyethylene-glycol (PEG) to induce DNA uptake. Transformed microcalli were selected in media containing G418 sulphate. NPT activity was detected in all antibiotic-resistant cell lines, but not all NPT-positive cell lines had GUS activity. Southern analysis confirmed the presence of sequences homologous to the NPT and GUS genes in DNA of G418-resistant callus.

引用

页码：437 / 444

页数：8

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